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n gruberi ckk  (Addgene inc)


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    Structured Review

    Addgene inc n gruberi ckk
    N Gruberi Ckk, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+gruberi+ckk/pDest+eGFP+rbFox1+(Plasmid+%2361278)/pm28991265-578-32-62
    Average 88 stars, based on 1 article reviews
    n gruberi ckk - by Bioz Stars, 2026-10
    88/100 stars

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    Modification:

    Article Title: A structural model for microtubule minus-end recognition and protection by CAMSAP proteins.
    Article Snippet: .. All proteins used for TIRFM (human CAMSAP1 CKK (residues 1474–1613) and CAMSAP1mini (residues 1227–1613), fly CKK (residues 1547–1689), worm CKK (residues 982–1128), T. thermophila CKK (residues 1635–1770), T. vaginalis CKK (residues 650–795), N. gruberi CKK (residues 612–788), P. infestans CKK (residues 1020– 1168), HsMCAK full length and human KIF5B (residues 1–560)) were expressed in HEK293T cells with a modified pTT5 expression vector (Addgene no. 44006 (ref. 54)) bearing Strep-GFP or Strep-SNAP tags at either the N or the C terminus of the protein. .. The cDNA sequences encoding T. thermophila, T. vaginalis and N. gruberi CKK were codon optimized for human-cell expression and synthesized as gBlocks by IDT.

    Expressing:

    Article Title: A structural model for microtubule minus-end recognition and protection by CAMSAP proteins.
    Article Snippet: .. All proteins used for TIRFM (human CAMSAP1 CKK (residues 1474–1613) and CAMSAP1mini (residues 1227–1613), fly CKK (residues 1547–1689), worm CKK (residues 982–1128), T. thermophila CKK (residues 1635–1770), T. vaginalis CKK (residues 650–795), N. gruberi CKK (residues 612–788), P. infestans CKK (residues 1020– 1168), HsMCAK full length and human KIF5B (residues 1–560)) were expressed in HEK293T cells with a modified pTT5 expression vector (Addgene no. 44006 (ref. 54)) bearing Strep-GFP or Strep-SNAP tags at either the N or the C terminus of the protein. .. The cDNA sequences encoding T. thermophila, T. vaginalis and N. gruberi CKK were codon optimized for human-cell expression and synthesized as gBlocks by IDT.

    Plasmid Preparation:

    Article Title: A structural model for microtubule minus-end recognition and protection by CAMSAP proteins.
    Article Snippet: .. All proteins used for TIRFM (human CAMSAP1 CKK (residues 1474–1613) and CAMSAP1mini (residues 1227–1613), fly CKK (residues 1547–1689), worm CKK (residues 982–1128), T. thermophila CKK (residues 1635–1770), T. vaginalis CKK (residues 650–795), N. gruberi CKK (residues 612–788), P. infestans CKK (residues 1020– 1168), HsMCAK full length and human KIF5B (residues 1–560)) were expressed in HEK293T cells with a modified pTT5 expression vector (Addgene no. 44006 (ref. 54)) bearing Strep-GFP or Strep-SNAP tags at either the N or the C terminus of the protein. .. The cDNA sequences encoding T. thermophila, T. vaginalis and N. gruberi CKK were codon optimized for human-cell expression and synthesized as gBlocks by IDT.



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    88
    Addgene inc n gruberi ckk
    N Gruberi Ckk, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+gruberi+ckk/pDest+eGFP+rbFox1+(Plasmid+%2361278)/pm28991265-578-32-62
    Average 88 stars, based on 1 article reviews
    n gruberi ckk - by Bioz Stars, 2026-10
    88/100 stars
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